Methylation & Metabolism · Document 07 of 24

MTHFR / Methylation

How Sample Subject's methylation-cycle variants are predicted to influence folate processing and homocysteine balance. All values below are synthetic.

3 findings1 Tier 1Reviewed 2026-01-15

Provenance

Data Provenanceverified chain

Document: MTHFR / Methylation
Source assembly: GRCh37 (hg19)
Source file SHA-256: 0000a1b2c3d4e5f6…4b5c6d7e
Supersedes: prior v1 · SHA 1111f0e9…
Evidence sources: CPIC, dbSNP, PubMed
Access date: 2026-01-15

Provider Alert

Considerations — Provider Ready3 items · discuss before supplementing

Consideration	Gene / Genotype	Evidence	Recommendation	Source
Folate form	MTHFR (`rs0067701` CT)	Strong	Reduced activity — discuss active 5-MTHF vs. folic acid with clinician.	PubMed ↗
Homocysteine	MTHFR / MTR	Moderate	Consider baseline homocysteine testing to contextualize findings.	PubMed ↗
B12 status	MTRR (`rs0067901` AG)	Limited	Monitor B12 if supplementing folate; combined support may be relevant.	dbSNP ↗

Per-Gene Findings

Filter:

MTHFR — Reduced Activity

Methylenetetrahydrofolate reductase · folate cycle

StrongTier 1

rs00677011:11,800,000CT

Sample Subject carries one reduced-activity allele, predicting roughly 60–70% of reference enzyme activity. This is associated with modestly elevated homocysteine and altered folate processing in some individuals.

Source: PubMed PMID 00067701 ↗ · dbSNP rs0067701 ↗

Historical contextPrior analysis (v1) reported this variant against an unspecified assembly; re-called here on GRCh37 with concordant genotype.

MTR — Normal Function

Methionine synthase · remethylation

ModerateTier 2

rs00678011:237,000,000AA

Reference genotype predicting normal methionine synthase function. No remethylation-pathway concern is indicated from this locus.

Source: dbSNP rs0067801 ↗

MTRR — Reduced Recycling

Methionine synthase reductase · B12 recycling

LimitedTier 2

rs00679015:7,900,000AG

Heterozygous genotype associated with mildly reduced B12 recycling. Clinical impact is uncertain and likely small in isolation.

Source: PubMed PMID 00067901 ↗

Genotype Table

Variants evaluated in this document — synthetic
rsID	chr:pos	Genotype	Gene	Significance
`rs0067701`	1:11,800,000	CT	MTHFR	Tier 1
`rs0067801`	1:237,000,000	AA	MTR	Tier 2
`rs0067901`	5:7,900,000	AG	MTRR	Tier 2

Consult your prescribing clinician. This HelixlyAI report is a synthetic demonstration generated from a consumer DNA export. Every name, identifier, rsID, genotype, and citation shown is fictional. It is not a diagnosis or medical advice — do not start, stop, or change any medication or treatment based on this document.